Trichoderma and Phosphite Elicited Distinctive Secondary Metabolite Signatures in Zucchini Squash Plants

Plant biostimulants are “green” solutions to improve crop production. Trichoderma spp. and phosphites, ordinarily used as biocontrol agents, can trigger phytostimulation, also promoting endogenous mechanisms involved in plant growth development. The present study aimed at assessing the efficacy of a phosphite-based formulation (Phosphit-One) harzianum-T22 on morpho-physiological response modulation metabolomics profile zucchini squash plants (Cucurbita pepo L.) cultivated controlled conditions (Fitotron®). highest values fresh biomass production (390.9 g plant?1) root dry weight (5.6 were obtained for Trichoderma-treated plants. This last treatment resulted an improved physiological performance (SPAD index, CO2 assimilation rate, Fv/Fm ratio) measured 30 days after transplanting. Both phosphite treatments induced broad metabolic reprogramming leaves, evident that did not result promotion. microbial non-microbial showed distinctive signatures secondary metabolism yet, common responses could be highlighted. For instance, both triggered ROS-mediated signaling processes, together with accumulation phenylpropanoids, glucosinolates, phytoalexins. Furthermore, significant alteration phytohormones was observed, terpenoid gibberellins brassinosteroids showing largest differences. metabolomic by provided molecular insights into processes underlying elicitation defense due biostimulation. Interestingly, impair growth.